广德6个竹种竹叶黄酮的抗氧化性研究.doc

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'广德6个竹种竹叶黄酮的抗氧化性研究.doc'
?广德6个竹种竹叶黄酮的抗氧化性研究  摘要:为优选出抗氧化性能优良的竹种,以斑苦竹[Pleioblastus maculatus C.D.Chu et C.S.Chao]、阔叶箬竹[Indocalamus latifolius Mc Clure]、黄秆乌哺鸡竹、雷竹、唐竹[Sinobambusa tootsik Makino]、茶秆竹[Pseudosasa amabilis Keng f.]6种竹的叶片为材料,以10倍原料重的60 %乙醇在70℃条件下,对竹叶进行总黄酮浸提,以芦丁为标准样品,采用分光光度法对提取的总黄酮进行了含量测定;采用烘箱贮存法测定竹叶黄酮在油脂中的抗氧化活性。结果表明,供试竹种的竹叶黄酮含量,以唐竹最低,为63.49 μg/mL,黄秆乌哺鸡竹的最高,为112.70 μg/mL。不同竹种的竹叶黄酮均具有一定程度的抗氧化效果,黄秆乌哺鸡竹的抗氧化作用最强,用其处理5 d的油脂体系的POV仅为38.06 meq/kg,较开始时增加了18.79 meq/kg,增加幅度为对照的31.5%;雷竹的抗氧化作用相对其他竹种较弱,处理5 d的POV增加幅度为对照的56.1%。因此黄秆乌哺鸡竹具有较大的开发潜力。   关键词:竹叶;黄酮;抗氧化性   中图分类号:S795;Q944.56;Q946.83 文献标识码:A文章编号:0439-811402-0318-03     。粒睿簦椋铮椋洌幔睿 Activities of Flavonoids Extracted from the Leaves  。铮 Six Bamboo Varieties in Guangde County     。龋粒 Pei-ying1,LIU Jing-jing2,YU Xiao-ping1        。粒猓螅簦颍幔悖: To select bamboo varieties with good antioxidant activities from Pleioblastus maculatus C.D. Chu et C.S.Chao, Indocalamus latifolius Mc Clure, Phyllostachys vivax Mc Clure cv. aureocanlis N.X.Ma, Phyllostachys praecox C.D.Chu et C.S.Chao, Sinobambusa tootsik Makino and Pseudosasa amabilis Keng f., leaves of the 6 bamboo varieties were extracted with the ethanol concentration 60%, temperature 70℃, solid -liquid ratio 1 g∶10 mL. Taking rutin as the standard substance, content of extracted flavonoids from bamboo leaves were determined by spectrophotometry. The antioxidant activity of extracted flavonoids to oil was evaluated by the Oven-Storage test. The results showed that the total flavonoids content in bamboo leaves ranged from 63.49 μg/mL to 112.70 μg/mL, and the highest concentration of flavonoids was found in Phyllostachys viax. cv. aureocaulis , while the lowest was in S. tootsik. Flavonoids extracted from all the 6 bamboo varieties showed some effect of anti-oxidation. Among the 6 varieties, Phyllostachys viax. cv. aureocaulis showed best anti-oxidant activity, and the peroxide value of the oil treated with this bamboo variety for 5 days was only 38.06 meq/kg, increased by 18.79 meq/kg in 5 days, which was just 31.5% of that of the blank control group. Phyllostachys praecox showed weakest anti-oxidant activity, and the increased POV was 56.1% of that of the blank control group. Phyllosltachys viax. cv. aureocaulis had good potential for further exploitation.  。耍澹 words: bamboo leaves; flavonoids; antioxidant activity      广德县是安徽省重点山区县之一,全县林业用地面积达12.6万hm2,占全县总面积的70%,其中竹林面积达5.7万hm2。1996年被原国家林业部命名为“中国竹子之乡”[1,2]。虽然竹林面积在不断扩大,但竹叶资源没有得到有效利用。竹叶在我国具有悠久的食用和药用历史,是中医一味著名的清热解毒药物[3]。现代研究表明,竹叶提取物中含有黄酮类化合物及苷类,具有优良的抗氧化、抗衰老、抗突变、抗菌、消炎、镇静、清热解毒、降压、利尿、防癌、抑制脂肪酶、;ば难艿壬镅ЧπВ郏矗罚。我们采用烘箱贮存法测定了竹叶黄酮在猪油中的抗氧化活性,考察了当地6个竹种的竹叶黄酮潜在的开发价值,以期为竹叶资源的开发利用提供理论依据。  。辈牧嫌敕椒  。保辈牧
关 键 词:
竹叶 广德 黄酮 抗氧化 研究
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